Journal article
MicroRNA genes and their target 3′-untranslated regions are infrequently somatically mutated in ovarian cancers
GL Ryland, JL Bearfoot, MA Doyle, SE Boyle, DYH Choong, SM Rowley, RW Tothill, KL Gorringe, IG Campbell
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2012
Abstract
MicroRNAs are key regulators of gene expression and have been shown to have altered expression in a variety of cancer types, including epithelial ovarian cancer. MiRNA function is most often achieved through binding to the 3′-untranslated region of the target protein coding gene. Mutation screening using massively-parallel sequencing of 712 miRNA genes in 86 ovarian cancer cases identified only 5 mutated miRNA genes, each in a different case. One mutation was located in the mature miRNA, and three mutations were predicted to alter the secondary structure of the miRNA transcript. Screening of the 3′-untranslated region of 18 candidate cancer genes identified one mutation in each of AKT2, EGFR..
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Awarded by U.S. Army Medical Research and Materiel Command
Funding Acknowledgements
This work was supported by the Victorian Breast Cancer Research Consortium, Australia, and the Australian National Health and Medical Research Council (NHMRC). GLR is supported by an Australian Postgraduate Award. The Australian Ovarian Cancer Study Group was supported by the U.S. Army Medical Research and Materiel Command under DAMD17-01-1-0729, The Cancer Council Tasmania, The Cancer Foundation of Western Australia and the NHMRC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.